CLS 296 - Clinical Practicum III

1. Perform automated blood cell counts on a minimum of 20 whole blood specimens. (counts must be within acceptable accuracy for method)
2. Perform a differential white blood cell count on 5 normal peripheral blood smears. (differential 80% accuracy; platelet estimate 100% accuracy; red blood cell morphology 100% accuracy)
3. Perform a differential white blood cell count on 5 abnormal peripheral blood smears. (differential 80% accuracy; platelet estimate 100% accuracy; red blood cell morphology 100% accuracy)
4. Perform 1 manual cell count using the hemacytometer. (+10% accuracy)
5. Perform a sickle cell screen, erythrocyte sedimentation rate, and a reticulocyte count on 1 whole blood specimen. (sickle cell screen 100% accuracy; ESR +5mm/hr accuracy; reticulocyte count +10% accuracy)
6. Determine prothrombin time and activated partial thromboplastin time on 5 whole blood specimens. (PT +0.5 seconds accuracy; APIT +1.5 seconds accuracy)
7. Determine fibrinogen concentration or another coagulation factor in 1 whole blood specimen using an automated instrument and procedure requiring the generation of a standard curve. (+10% accuracy)
8. Prepare a 1:4, 1:10, and 1:20 dilution of 1 serum specimen; then perform two-fold serial dilutions from the 1:20 dilution to include the following dilutions: 1:40, 1:80, 1:160, 1:320. (proper pipetting technique; 100% accuracy)
9. Perform and interpret qualitative and quantitative RPR/VDRL test on 15 serum specimens. (100% accuracy; RF titer +1 dilution)
10. Perform and interpret qualitative and quantitative test for RF factor on 10 serum specimens. (RF screen 100% accuracy)
11. Perform and interpret qualitative tests for rubella antibodies on 10 serum specimens. (100% accuracy)
12. Perform and interpret mono-spot test on 10 serum specimens. (100% accuracy)
13. Perform and interpret cold agglutinin test on 5 serum specimens. (100% accuracy)
14. Determine the ABO/Rh of 20 patients and determine the presence of atypical antibodies in the patients serum by performing the indirect antiglobulin test. (100% accuracy)
15. Perform major crossmatches of donor and recipient blood. (100% accuracy; correct “compatible/incompatible” interpretation)
16. Perform direct antiglobulin test and interpret the results. (100% accuracy)
17. Identify a single antibody in a serum specimen using a reagent red blood cell panel. (acceptable technique; correct identification)
18. Identify multiple antibodies in a serum specimen using a reagent red blood cell panel. (acceptable technique; correct identification)
19. Perform an elution on a sensitized blood specimen. (correct removal of antibody from RBCs)
20. Perform RhIg pre-administration testing and interpret the results. (all necessary tests performed; 100% accuracy)